3OT6
Crystal Structure of an enoyl-CoA hydratase/isomerase family protein from Psudomonas syringae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 162.517, 162.517, 162.517 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 114.710 - 2.500 |
| R-factor | 0.18951 |
| Rwork | 0.187 |
| R-free | 0.25164 |
| Structure solution method | SAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.869 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 114.710 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 23759 |
| Completeness [%] | 99.0 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.20M ammonium phosphate, 0.10M tris pH 8.5, 50% v/v MPD (2-methyl-1,3 propanediol), 2% v/v dioxane, 0.1mM n-octyl-beta-D-thioglucoside, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
