3OT1
Crystal structure of VC2308 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-09 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.387, 64.683, 81.592 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.160 |
| R-factor | 0.1448 |
| Rwork | 0.144 |
| R-free | 0.16775 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.500 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 (MLPHARE) |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.180 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.094 | 0.610 |
| Number of reflections | 115862 | |
| <I/σ(I)> | 23.8 | 1.9 |
| Completeness [%] | 97.8 | 84.5 |
| Redundancy | 9.12 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Tacsimate 35%, Phenol 10mM, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
