3OSZ
Crystal Structure of the complex of proteinase K with an antimicrobial nonapeptide, at 2.26 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-04-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.514 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 68.290, 68.290, 108.350 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.400 - 2.260 |
| R-factor | 0.166 |
| Rwork | 0.166 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p7v |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.500 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.700 | 2.310 |
| High resolution limit [Å] | 2.260 | 2.260 |
| Number of reflections | 12587 | |
| <I/σ(I)> | 5.7 | 2.1 |
| Completeness [%] | 97.1 | 96.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | tris HCl, CaCl2, NaNO3, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
