3OSV
The crytsal structure of FLGD from P. Aeruginosa
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Detector technology | CCD |
| Collection date | 2009-02-10 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9790, 0.9793 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 114.828, 118.379, 118.301 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.070 - 2.350 |
| R-factor | 0.214 |
| Rwork | 0.211 |
| R-free | 0.26800 |
| Structure solution method | MAD |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.996 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.345 | 2.350 |
| Rmerge | 0.069 | 0.069 |
| Number of reflections | 34082 | |
| <I/σ(I)> | 5 | 0.026 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.7 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.6 | 293 | 0.1M MES, PH 6.60-6.80, 20% PEG 6000, VAPOR DIFFUSION, temperature 293K |
