3OST
Structure of the Kinase Associated-1 (KA1) from Kcc4p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.373, 59.559, 37.566 |
| Unit cell angles | 90.00, 91.84, 90.00 |
Refinement procedure
| Resolution | 26.333 - 1.694 |
| R-factor | 0.1525 |
| Rwork | 0.151 |
| R-free | 0.18560 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.144 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELX (C/D/E) |
| Refinement software | PHENIX (1.6_289) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.740 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Number of reflections | 14366 | |
| <I/σ(I)> | 51.8 | |
| Completeness [%] | 97.9 | 83.2 |
| Redundancy | 6 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 294 | 0.1 M HEPES, pH 7.4, 0.2 M (NH4)2SO4 plus 20% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
