3OSH
Crystal Structure of The Complex of Group 1 Phospholipase A2 With Atropin At 1.5 A Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-05-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 41 |
| Unit cell lengths | 42.377, 42.377, 65.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.370 - 1.500 |
| R-factor | 0.20078 |
| Rwork | 0.199 |
| R-free | 0.22945 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nju |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.174 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 18066 | |
| <I/σ(I)> | 73.6 | 2.4 |
| Completeness [%] | 97.0 | 91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 10MM sodium phosphate buffer, 2M calcium chloride, 35% ethanol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
