3OSE
Structure of the Kinase Associated Domain 1 (KA1) from MARK1 kinase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-16 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 69.843, 69.843, 54.446 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.1759 |
| Rwork | 0.175 |
| R-free | 0.19452 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.238 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Number of reflections | 18599 | |
| <I/σ(I)> | 29.8 | |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 7.2 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 294 | 0.1 M Na acetate, pH 4.6, 0.04 M CaCl2, and 15-25% (w/v) PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
