3ORU
Crystal structure of a DUF1989 family protein (TM1040_0329) from SILICIBACTER SP. TM1040 at 1.11 A resolution
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-12 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91837,0.97981,0.97944 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 61.757, 131.024, 57.902 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.951 - 1.110 |
| R-factor | 0.1274 |
| Rwork | 0.126 |
| R-free | 0.14650 |
| Structure solution method | MAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.780 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 28.951 | 1.150 | |
| High resolution limit [Å] | 1.110 | 2.390 | 1.110 |
| Rmerge | 0.056 | 0.035 | 0.375 |
| Number of reflections | 92236 | 17919 | 17211 |
| <I/σ(I)> | 11.09 | 37.2 | 2.1 |
| Completeness [%] | 99.1 | 99.6 | 95.4 |
| Redundancy | 6.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 22.0% PEG 3350, 0.10M Mg Cl, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
