3ORG

Crystal Structure of a eukaryotic CLC transporter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Detector technology	CCD
Collection date	2009-08-07
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	229.629, 178.270, 145.135
Unit cell angles	90.00, 129.21, 90.00

Refinement procedure

Resolution	29.660 - 3.500
R-factor	0.2606
Rwork	0.259
R-free	0.28410
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.246
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.630
High resolution limit [Å]	3.500	7.530	3.500
Rmerge	0.091	0.054	0.855
Number of reflections	56764
<I/σ(I)>	10.2
Completeness [%]	99.7	97.4	100
Redundancy	5.1	4.8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.7	298	PEG 400, 200mM ammonium sulfate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.7	298	PEG 400, 200mM ammonium sulfate, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298K