3OR3
Restriction endonuclease HPY188I in complex with product DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.281 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.395, 65.395, 221.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.950 |
| R-factor | 0.17013 |
| Rwork | 0.168 |
| R-free | 0.20144 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oqg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.281 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.068 | 0.406 |
| Number of reflections | 35348 | |
| <I/σ(I)> | 37.775 | 6.488 |
| Completeness [%] | 97.5 | 95.8 |
| Redundancy | 13.99 | 14.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 292 | 0.1 M MES/NaOH pH 6.2 and 30% MPD. CRYSTAL WAS SOAKED IN 0.1 M CaCl2 FOR 18 H., VAPOR DIFFUSION, SITTING DROP, temperature 292K |
