3OQM
structure of ccpa-hpr-ser46p-ackA2 complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-12-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 74.240, 103.540, 176.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.100 - 2.960 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.27100 |
| Structure solution method | MIR |
| Starting model (for MR) | 2fep |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 103.700 | 3.050 |
| High resolution limit [Å] | 2.960 | 2.960 |
| Number of reflections | 28736 | |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | PEG 8000, Ammonium sulphate, Mes, magnesium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
