3OQH
Crystal structure of B. licheniformis CDPS yvmC-BLIC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-02-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.719, 54.911, 101.523 |
Unit cell angles | 90.00, 130.66, 90.00 |
Refinement procedure
Resolution | 37.413 - 1.901 |
R-factor | 0.1811 |
Rwork | 0.180 |
R-free | 0.20510 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.977 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
Rmerge | 0.092 | 0.031 | 0.692 |
Number of reflections | 49609 | ||
<I/σ(I)> | 6.8 | ||
Completeness [%] | 99.8 | 99.6 | 100 |
Redundancy | 3.7 | 3.7 | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 20% PEG 8000, pH 9.5, vapor diffusion, hanging drop, temperature 293K |