3OQG
Restriction endonuclease HPY188I in complex with substrate DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-10 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.05 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 65.236, 65.236, 220.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.900 - 1.750 |
| R-factor | 0.16612 |
| Rwork | 0.165 |
| R-free | 0.19218 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.224 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXCD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.770 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.061 | 0.440 |
| Number of reflections | 48330 | |
| <I/σ(I)> | 24.651 | 3.265 |
| Completeness [%] | 98.0 | 97.1 |
| Redundancy | 5.55 | 5.55 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 292 | 0.1 M MES/NaOH pH 6.2 and 30% MPD, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
