3OQD
Crystal Structures of Multidrug-Resistant Clinical Isolate 769 HIV-1 Protease Variants
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-09 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 44.968, 44.968, 104.959 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.620 - 1.710 |
| R-factor | 0.19768 |
| Rwork | 0.196 |
| R-free | 0.22920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.333 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.620 | 1.800 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Number of reflections | 12231 | |
| <I/σ(I)> | 2.8 | |
| Completeness [%] | 100 | |
| Redundancy | 11.3 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 0.3-1.0M sodium chloride in the pH range 5.5-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
