3OPO
Crystal structure of the membrane fusion protein CusB from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.3779 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 84.803, 114.678, 259.166 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.310 - 3.848 |
| R-factor | 0.2971 |
| Rwork | 0.295 |
| R-free | 0.33970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.962 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.980 |
| High resolution limit [Å] | 3.840 | 3.840 |
| Number of reflections | 12345 | |
| Completeness [%] | 99.4 | 95.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | PEG1000 15%, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
