3OPN
The crystal structure of a putative hemolysin from Lactococcus lactis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.716, 54.523, 70.643 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.162 - 2.050 |
| R-factor | 0.1822 |
| Rwork | 0.179 |
| R-free | 0.23460 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.640 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.062 | 0.117 |
| Number of reflections | 12358 | |
| <I/σ(I)> | 33 | 19.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.9 | 13.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.2 M Ammonium sulfate, 0.1 M cacodylate trihydrate pH 6.5, 30% w/v PEG 400, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
