3OPK
Crystal structure of divalent-cation tolerance protein CutA from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-08-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.333, 107.925, 52.177 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.17744 |
| Rwork | 0.175 |
| R-free | 0.21867 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gsd |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.867 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.106 | 0.514 |
| Number of reflections | 28212 | |
| <I/σ(I)> | 13.7 | 2 |
| Completeness [%] | 99.4 | 95.7 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 2.8 M sodium Acetate, 10mM ADP, 1mM Magnesium chloride, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
