3OP6

Crystal structure of an oligo-nucleotide binding protein (lpg1207) from Legionella pneumophila subsp. pneumophila str. philadelphia 1 at 2.00 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-23
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.91837,0.97927,0.97913
Spacegroup name	P 21 21 21
Unit cell lengths	54.005, 63.828, 84.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.877 - 2.000
R-factor	0.1969
Rwork	0.195
R-free	0.23080
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SHELX
Refinement software	BUSTER-TNT (BUSTER 2.8.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.877	29.877	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge		0.041	0.747
Total number of observations		808	5427
Number of reflections	20475
<I/σ(I)>	7.3	13.5	1
Completeness [%]	99.9	96.2	100
Redundancy	3.6	3	3.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.4000M Na3Citrate, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K