3OOX
Crystal structure of a putative 2OG-Fe(II) oxygenase family protein (CC_0200) from CAULOBACTER CRESCENTUS at 1.44 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-03-11 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.645, 80.920, 142.373 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.822 - 1.440 |
R-factor | 0.1704 |
Rwork | 0.169 |
R-free | 0.19690 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.391 |
Data reduction software | MOSFLM |
Data scaling software | SCALA (3.3.15) |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.822 | 28.822 | 1.480 |
High resolution limit [Å] | 1.440 | 6.440 | 1.440 |
Rmerge | 0.042 | 0.927 | |
Total number of observations | 6445 | 42297 | |
Number of reflections | 118773 | ||
<I/σ(I)> | 9.3 | 21.7 | 1.7 |
Completeness [%] | 98.4 | 96.2 | 96.8 |
Redundancy | 5 | 4.4 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 20.0% polyethylene glycol 4000, 5.0% 2-propanol, 0.1M HEPES pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |