3OON
The structure of an outer membrance protein from Borrelia burgdorferi B31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-09 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 52.089, 74.784, 66.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.740 - 1.790 |
| R-factor | 0.2136 |
| Rwork | 0.212 |
| R-free | 0.23614 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.147 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.800 |
| Rmerge | 0.069 | 0.210 |
| Number of reflections | 12430 | |
| <I/σ(I)> | 50.6 | 7.35 |
| Completeness [%] | 98.4 | 100 |
| Redundancy | 5.3 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.20M ammonium sulfate, 0.1M bis-tris, 25%w/v PEG3350, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
