3ONT
The Crystal Structure of Spot14, a modulator of lipogenesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | I 4 3 2 |
| Unit cell lengths | 144.951, 144.951, 144.951 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.740 - 2.650 |
| R-factor | 0.22424 |
| Rwork | 0.223 |
| R-free | 0.24912 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.389 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Number of reflections | 7919 | |
| <I/σ(I)> | 14.9 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.1 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 0.100 M MES pH 6.0, 0.015 M magnesium chloride, 5% (w/v) polyethylene glycol 3000 and 30% (v/v) polyethylene glycol 200, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
