3ONL

yeast Ent3_ENTH-Vti1p_Habc complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2010-01-15
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	58.683, 82.351, 95.953
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.710 - 2.200
R-factor	0.2256
Rwork	0.223
R-free	0.26930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3ONJ and 3ONK
RMSD bond length	0.008
RMSD bond angle	1.051
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Number of reflections	23733
Completeness [%]	98.4	98.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		288	0.2M tri-Lithium Citrate tetrahydrate, 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 288.0K