3ONK
yeast Ent3_ENTH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-09-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.600, 37.589, 38.109 |
| Unit cell angles | 77.81, 86.64, 65.54 |
Refinement procedure
| Resolution | 33.480 - 2.090 |
| R-factor | 0.2015 |
| Rwork | 0.195 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xgw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.027 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.180 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 7327 | |
| Completeness [%] | 95.1 | 93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 288 | 8% tacsimate, 20% PEG 3350, pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
