3ONA
The SECRET domain in complex with CX3CL1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 71.330, 71.330, 93.141 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.317 - 2.600 |
| R-factor | 0.1986 |
| Rwork | 0.196 |
| R-free | 0.24970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3ON9 AND 1F2L |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 8847 | |
| Completeness [%] | 99.9 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2M Li2SO4, 0.1M Tris.Cl, 20% PEG 4000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
