3OMY
Crystal structure of the pED208 TraM N-terminal domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1158 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 54.069, 54.069, 67.268 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.140 - 1.300 |
| R-factor | 0.15092 |
| Rwork | 0.150 |
| R-free | 0.17293 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cmb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.380 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.350 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 25159 | |
| <I/σ(I)> | 39.2 | 6.6 |
| Completeness [%] | 81.0 | 100 |
| Redundancy | 7.5 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 5% PEG 4000, 50mM MES, 5mM magnesium sulphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
