3OMX
Crystal structure of Ssu72 with vanadate complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-24 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 157.616, 102.309, 65.816 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.656 - 2.337 |
| R-factor | 0.2078 |
| Rwork | 0.205 |
| R-free | 0.26730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3omw |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.914 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.670 | 2.390 |
| High resolution limit [Å] | 2.337 | 2.337 |
| Number of reflections | 45695 | |
| <I/σ(I)> | 36.5 | 1.5 |
| Completeness [%] | 99.9 | 98.4 |
| Redundancy | 6.6 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.1M HEPES pH 7.5, 10% isopropanol, 14-18% PEG4000, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K |
