3OMG

Structure of human SND1 extended tudor domain in complex with the symmetrically dimethylated arginine PIWIL1 peptide R14me2s

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-30
Detector	RIGAKU SATURN A200
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	35.721, 75.804, 80.073
Unit cell angles	90.00, 90.36, 90.00

Refinement procedure

Resolution	80.070 - 1.850
R-factor	0.1943
Rwork	0.192
R-free	0.23860
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2o4x
RMSD bond length	0.011
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC (refmac_5.6.0081)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.070	50.000	1.920
High resolution limit [Å]	1.850	3.990	1.850
Rmerge	0.084	0.037	0.448
Number of reflections	35716
<I/σ(I)>	9.9
Completeness [%]	97.9	99.7	85.9
Redundancy	5.2	5.5	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	30% PEG1500, 1:500 V8 protease, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K