3OLZ
Crystal structure of the GluK3 (GluR7) ATD dimer at 2.75 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | P 61 |
| Unit cell lengths | 171.211, 171.211, 68.234 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.307 - 2.750 |
| R-factor | 0.1978 |
| Rwork | 0.195 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h6g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.956 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.850 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.091 | 0.640 |
| Number of reflections | 29967 | |
| <I/σ(I)> | 16 | 2.25 |
| Completeness [%] | 98.0 | 97.3 |
| Redundancy | 4.5 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 277 | 0.1 M MgCl2, 0.1 M MES, 10% Isopropanol, 5% PEG 4K, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
