3OLQ
The crystal structure of a universal stress protein E from Proteus mirabilis HI4320
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 108.655, 108.655, 74.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.360 - 1.816 |
| R-factor | 0.1724 |
| Rwork | 0.171 |
| R-free | 0.19610 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.996 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.500 | 1.850 |
| High resolution limit [Å] | 1.816 | 1.820 |
| Rmerge | 0.093 | 0.810 |
| Number of reflections | 40612 | |
| <I/σ(I)> | 31.6 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | 0.4M NaH2PO4/1.6M K2HPO4, 0.1M imidazole, 0.2M NaCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
