3OLJ
Crystal structure of human ribonucleotide reductase subunit M2 (hRRM2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 173 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-12-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.908, 109.725, 174.056 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.900 - 2.100 |
| R-factor | 0.20102 |
| Rwork | 0.199 |
| R-free | 0.23711 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w69 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.092 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.051 | 0.546 |
| Number of reflections | 118751 | |
| <I/σ(I)> | 23.8 | 1.36 |
| Completeness [%] | 96.2 | 78.6 |
| Redundancy | 7.1 | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | 1.4M NaH2PO4/K2HPO4, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K |
