3OLC
Crystal structure of the N-terminal region of TopBP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 115.730, 115.730, 115.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 81.833 - 2.400 |
| R-factor | 0.22267 |
| Rwork | 0.221 |
| R-free | 0.25787 |
| Structure solution method | SAD |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.952 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXS |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.833 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.078 | 0.078 |
| Number of reflections | 20506 | |
| <I/σ(I)> | 27.9 | 4.95 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M MES, 11%(w/v) PEG3350, 0.85M sodium nitrate, 2mM EDTA, 4mM DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
