3OKS

Crystal structure of 4-aminobutyrate transaminase from mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97740
Spacegroup name	P 43
Unit cell lengths	128.010, 128.010, 104.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.200 - 1.800
R-factor	0.128
Rwork	0.126
R-free	0.15900
Structure solution method	MR, MR
RMSD bond length	0.015
RMSD bond angle	1.456
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.200	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.109	0.477
Number of reflections	155089
<I/σ(I)>	14.91	4
Completeness [%]	100.0	100
Redundancy	7.35	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.9	289	JCSG SCREEN CONDITION A5, TRAY BARCODE 216078: 200MM MG FORMATE PH 5.9, 20% PEG 3350, MYSMA.01026.C.A1 AT 25.11 MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 289K