3OK2

Crystal structure of the ANA:RNA decamer without lattice translocation defects

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	26.070, 42.510, 157.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.450 - 1.963
R-factor	0.22443
Rwork	0.224
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	2.508
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.450	39.447	2.070
High resolution limit [Å]	1.960	6.210	1.960
Number of reflections	11922
<I/σ(I)>	9.3
Completeness [%]	90.3	74.045	93.7
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.01M NiCl2, 1.0M Li2SO4, 0.1M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K