3OJD
Anti-Indolicidin monoclonal antibody V2D2 (Fab fragment)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-08-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.190, 63.710, 164.020 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.500 - 2.000 |
| Rwork | 0.205 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ymh |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.500 |
| Data reduction software | AUTOMAR |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 1.960 |
| Rmerge | 0.113 | 0.526 |
| Number of reflections | 28494 | |
| <I/σ(I)> | 10.5 | 3 |
| Completeness [%] | 90.0 | 75.3 |
| Redundancy | 5.5 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 10% PEG 1000, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
