3OJB
Crystal structure of C-terminal domain of human galectin-8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-29 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 2 21 |
| Unit cell lengths | 54.105, 73.148, 179.214 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.010 |
| R-factor | 0.23129 |
| Rwork | 0.228 |
| R-free | 0.29190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yv8 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.214 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.006 | 3.000 |
| Rmerge | 0.219 | 0.413 |
| Number of reflections | 13191 | |
| Completeness [%] | 88.9 | 67.1 |
| Redundancy | 5.49 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 281 | 27.5% PEG 8000, 0.2M ammonium phosphate, 6.5% Ethylene Glycol, 0.1M Na Cacodylate, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
