3OJ0
Crystal structure of glutamyl-tRNA reductase from Thermoplasma volcanium (nucleotide binding domain)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9791831 |
| Spacegroup name | P 61 |
| Unit cell lengths | 92.520, 92.520, 33.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.687 - 1.648 |
| R-factor | 0.1608 |
| Rwork | 0.160 |
| R-free | 0.18330 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.298 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.648 | 1.650 |
| Rmerge | 0.068 | 0.572 |
| Number of reflections | 19834 | |
| <I/σ(I)> | 28.4 | 2.2 |
| Completeness [%] | 98.8 | 99.5 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 289 | 0.1 M sodium acetate, 2.0 ammonium sulfate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
