3OIZ
Crystal structure of antisigma-factor antagonist, STAS domain from Rhodobacter sphaeroides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97935 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 72.102, 72.102, 36.429 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.650 |
| R-factor | 0.1623 |
| Rwork | 0.160 |
| R-free | 0.21560 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.420 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.097 | 0.507 |
| Number of reflections | 12047 | |
| <I/σ(I)> | 62.2 | 2.9 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 10.5 | 4.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 297 | 0.2M Calcium acetate, 0.1M MES, 10% iso-propanol, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
