3OIY
Helicase domain of reverse gyrase from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-02-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.646, 126.514, 132.707 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.358 - 2.350 |
| R-factor | 0.2105 |
| Rwork | 0.208 |
| R-free | 0.25110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gku |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.564 |
| Data reduction software | XDS ((VERSION May 10) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 132.710 | 2.440 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.099 | 0.659 |
| Number of reflections | 42547 | |
| <I/σ(I)> | 9.7 | 1.34 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 7.1 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.2 M Magnesium formiate, 20% PEG 3350 |
