3OIU
H-RasQ61L with allosteric switch in the "on" state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 87.985, 87.985, 135.146 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.886 - 1.320 |
| R-factor | 0.1821 |
| Rwork | 0.182 |
| R-free | 0.19230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.199 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.1_336) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.320 |
| High resolution limit [Å] | 1.300 | 3.530 | 1.300 |
| Rmerge | 0.103 | 0.060 | |
| Number of reflections | 48665 | ||
| <I/σ(I)> | 14.7 | ||
| Completeness [%] | 99.8 | 96.9 | 99.9 |
| Redundancy | 9.7 | 9.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | sitting drop | 7.5 | 298 | 365mM Calcium Acetate, 24 % PEG 3350, pH 7.5, sitting drop, temperature 298K |
