3OIP

Crystal structure of Yeast telomere protein Cdc13 OB1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-12-21
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 2
Unit cell lengths	62.515, 68.641, 52.815
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.781 - 2.504
R-factor	0.2086
Rwork	0.203
R-free	0.25680
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.198
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000
High resolution limit [Å]	2.500
Number of reflections	7955
Completeness [%]	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	21% PEG 3350, 0.2M NaCl, 0.1M HEPES, 10mM DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K