3OIH
Crystal Structure of the complex of xylanase-alpha-amylase inhibitor Protein (XAIP-I) with trehalose at 1.87 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2010-05-13 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.238, 64.677, 48.852 |
| Unit cell angles | 90.00, 102.33, 90.00 |
Refinement procedure
| Resolution | 47.730 - 1.870 |
| R-factor | 0.18687 |
| Rwork | 0.186 |
| R-free | 0.21073 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hu7 |
| RMSD bond length | 0.026 |
| RMSD bond angle | 1.792 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.730 | 1.900 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 20087 | |
| <I/σ(I)> | 39.5 | 5.1 |
| Completeness [%] | 99.4 | 96.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 298 | 0.1M ammonium sulphate, 20% PEG 6000, 0.1M sodium acetate, 20mM phosphate buffer, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
