3OHW

X-Ray Structure of Phycobilisome LCM core-membrane linker polypeptide (fragment 721-860) from Synechocystis sp. PCC 6803, Northeast Structural Genomics Consortium Target SgR209E

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-10
Detector	ADSC QUANTUM 4r
Wavelength(s)	0.979
Spacegroup name	P 1 21 1
Unit cell lengths	29.891, 74.881, 66.388
Unit cell angles	90.00, 91.51, 90.00

Refinement procedure

Resolution	27.753 - 2.700
R-factor	0.24
Rwork	0.237
R-free	0.26600
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.346
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.700
Rmerge	0.112
Number of reflections	15697
<I/σ(I)>	10.9
Completeness [%]	98.0
Redundancy	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	macrobatch under oik	7	297	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution:tryptone 1% (w/v), HEPES sodium 0.05M, PEG3350 20% (w/v), macrobatch under oik, temperature 297K