3OHU
Crystal structure of the human Bach2 POZ domain, form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.600, 96.970, 82.630 |
| Unit cell angles | 90.00, 101.06, 90.00 |
Refinement procedure
| Resolution | 40.550 - 2.100 |
| R-factor | 0.21231 |
| Rwork | 0.210 |
| R-free | 0.25661 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ihc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.259 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.570 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.093 | 0.726 |
| Number of reflections | 49842 | |
| <I/σ(I)> | 9.2 | 2.9 |
| Completeness [%] | 99.2 | 99.7 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | 0.2 M ammonium sulphate 0.1 M MES pH 5.5 4% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
