3OH4

Protein structure of USP from L. major bound to URIDINE-5'-DIPHOSPHATE Glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9814
Spacegroup name	C 1 2 1
Unit cell lengths	107.814, 121.665, 61.393
Unit cell angles	90.00, 105.73, 90.00

Refinement procedure

Resolution	32.891 - 2.210
R-factor	0.18
Rwork	0.178
R-free	0.21240
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3ogz
RMSD bond length	0.005
RMSD bond angle	0.881
Data scaling software	SCALEPACK
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.300
High resolution limit [Å]	2.210	4.780	2.210
Rmerge	0.051	0.025	0.502
Number of reflections	37828
<I/σ(I)>	15.8
Completeness [%]	99.7	98.4	98.4
Redundancy	4.4	4.4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1