3OH2
Protein structure of USP from L. major bound to URIDINE-5'-DIPHOSPHATE-GALACTOSE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.433, 121.910, 61.407 |
| Unit cell angles | 90.00, 105.98, 90.00 |
Refinement procedure
| Resolution | 29.517 - 2.140 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.20340 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ogz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.220 |
| High resolution limit [Å] | 2.140 | 4.610 | 2.140 |
| Rmerge | 0.051 | 0.038 | 0.359 |
| Number of reflections | 41738 | ||
| <I/σ(I)> | 22 | ||
| Completeness [%] | 99.1 | 93.3 | 100 |
| Redundancy | 4.2 | 4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1-0.2 M sodium tartrate, 16-20% PEG5000, 10 mM DTT, pH 7-8, vapor diffusion, sitting drop, temperature 292K |
