3OH0
Protein structure of USP from L. major bound to URIDINE-5'-TRIPHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-22 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.880, 122.482, 60.851 |
| Unit cell angles | 90.00, 105.22, 90.00 |
Refinement procedure
| Resolution | 19.722 - 2.150 |
| R-factor | 0.1974 |
| Rwork | 0.196 |
| R-free | 0.22930 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3ogz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.049 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 | |
| High resolution limit [Å] | 2.150 | 30.000 | 2.150 |
| Rmerge | 0.043 | 0.546 | |
| Number of reflections | 40870 | 5203 | |
| <I/σ(I)> | 24.84 | 3 | |
| Completeness [%] | 99.5 | 99.8 | |
| Redundancy | 4.26 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1-0.2 M sodium tartrate, 16-20% PEG5000, 10 mM DTT, pH 7-8, vapor diffusion, sitting drop, temperature 292K |
