3OGZ

Protein structure of USP from L. major in Apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-11-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	107.392, 123.196, 61.342
Unit cell angles	90.00, 104.32, 90.00

Refinement procedure

Resolution	29.741 - 2.030
R-factor	0.1861
Rwork	0.184
R-free	0.22000
Structure solution method	SIRAS
RMSD bond length	0.005
RMSD bond angle	0.843
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.100
High resolution limit [Å]	2.030	4.370	2.030
Rmerge	0.044	0.022	0.508
Number of reflections	49970
<I/σ(I)>	16.8
Completeness [%]	99.7	97.3	100
Redundancy	4.4	4.3	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1-0.2 M sodium tartrate, 16-20% PEG5000, 10 mM DTT, pH 7-8, vapor diffusion, sitting drop, temperature 292K