3OGN

Crystal Structure of an Odorant-binding Protein from the Southern House Mosquito Complexed with an Oviposition Pheromone

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-14
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1 21 1
Unit cell lengths	35.915, 107.310, 38.529
Unit cell angles	90.00, 100.38, 90.00

Refinement procedure

Resolution	26.830 - 1.300
R-factor	0.13415
Rwork	0.132
R-free	0.17374
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.029
RMSD bond angle	2.366
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.5.0102)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.350
High resolution limit [Å]	1.300	2.800	1.300
Number of reflections	68116
Completeness [%]	96.9	91.9	95.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	298.15	20% (wt/vol) PEG 4,000, 100 mM HEPES, 200 mM MgCl2, pH 8.2, VAPOR DIFFUSION, HANGING DROP, temperature 298.15K