3OGJ

Crystal structure of partial apo (92-227) of cGMP-dependent protein kinase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-05-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 43
Unit cell lengths	62.588, 62.588, 199.522
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.256 - 2.751
R-factor	0.1834
Rwork	0.180
R-free	0.25140
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.942
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	2.750
Rmerge	0.045
Number of reflections	19584
<I/σ(I)>	31.17
Completeness [%]	99.9
Redundancy	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.1	295.15	1.4 M sodium/potassium phosphate, pH 8.1, VAPOR DIFFUSION, temperature 295.15K