3OGI

Crystal structure of the Mycobacterium tuberculosis H37Rv EsxOP complex (Rv2346c-Rv2347c)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-04-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918, 0.97938
Spacegroup name	P 32 2 1
Unit cell lengths	66.383, 66.383, 162.610
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.307 - 2.549
R-factor	0.2181
Rwork	0.216
R-free	0.26000
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.067
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.640
High resolution limit [Å]	2.550	2.550
Rmerge	0.078	0.449
Number of reflections	25291
<I/σ(I)>		3.4
Completeness [%]	100.0	96.5
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	Reservoir: 9.0% isopropanol, 200 mM CaCl2, 90 mM sodium acetate trihydrate pH 4.6. Protein buffer: 50 mM HEPES pH 7.8, 150 mM NaCl. Ratio = 2:1 protein solution to reservoir solution, VAPOR DIFFUSION, HANGING DROP, temperature 293K